Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance.
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چکیده
منابع مشابه
Amino acid changes linked to pyrimethamine resistance in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum.
We describe the isolation and the sequence of the gene for the bifunctional enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS; EC 1.5.1.3 and EC 2.1.1.45, respectively) from two pyrimethamine-resistant clones of Plasmodium falciparum, HB3 and 7G8. We have also derived the sequence of the DHFR portion of the gene, by amplification using polymerase chain reaction, for the pyrimethamine...
متن کاملmolecular characterization of dihydrofolate reductase-thymidylate synthase gene concerning antifulate resistance of plasmodium vivax
background: the recently reported resistance to antimalarials contributes to making the control of malaria more difficult. there is a need to evaluate the current antimalaria regimens to prevent this emerging problem. the aim of this study was to determine dihydrofolate reductase-thymidylate synthase gene mutation ( pvdhfr ) regarding antifulate resistance in plasmodium vivax . methods : from...
متن کامل"pyrimethamine and proguanil resistance-conferring key mutations in dihydrofolate reductase gene of plasmodium falciparum isolates from iran"
using mutation specific pcr assay and analysis of position 108 amino acid in dihydrofolate reductase-thymidilate synthase gene in 70 plasmodium falciparum isolates of malarial patients from southern areas of iran, showed 4 different situations: iwild type (108-ser) ii-mutant type (108-thr) iii-mutant type (108-asn) and iv-mixed wild and mutant type (108-ser+thr) with frequency of 58(82.8%), 2(2...
متن کاملEvidence that a point mutation in dihydrofolate reductase-thymidylate synthase confers resistance to pyrimethamine in falciparum malaria.
Analysis of a genetic cross of Plasmodium falciparum and of independent parasite isolates from Southeast Asia, Africa, and South America indicates that resistance to pyrimethamine, an antifolate used in the treatment of malaria, results from point mutations in the gene encoding dihydrofolate reductase-thymidylate synthase (EC 1.5.1.3 and EC 2.1.1.45, respectively). Parasites having a mutation f...
متن کاملCharacteristics of genetic hitchhiking around dihydrofolate reductase gene associated with pyrimethamine resistance in Plasmodium falciparum isolates from India.
Sulfadoxine-pyrimethamine (SP) resistance in Plasmodium falciparum has been widespread across continents, causing the major hurdle of controlling malaria. Resistance is encoded mainly by point mutations in P. falciparum dihydrofolate reductase (pfdhfr) and dihydropteroate synthase (pfdhps) target genes. To study the origin and evolution of pyrimethamine resistance on the Indian subcontinent, mi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.8.3014